Coding

Part:BBa_K2695011:Design

Designed by: Nahnsu Dawkins   Group: iGEM18_Exeter   (2018-10-17)


T7 pcrBCD from A.suillum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to large size of pcrABCD, we split it up into two parts. This pcrBCD part was assembled with pcrA using biobrick assembly to form pcrABCD.


Source

Amino acid sequence translated from Dsui_0148(PcrB), Dsui_0147(PcrC), Dsui_0146(PcrD) from Azospira suillum PS Genome, GenBank CP003153

References